Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody
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- Target
- Melanoma Marker (MART-1 + Tyrosinase + Gp100)
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
- Specificity
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant hMART-1 protein (M2-7C10, M2-9E3), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
- Clone
- M2-7C10-M2-9E3-T311-HMB45
- Isotype
- IgG1 kappa
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anti-Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody
Reactivity: Human IHC, ISt, StM Host: Mouse Monoclonal DT101-BC199-T311-HMB45 unconjugated
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- Application Notes
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Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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