eIF4EBP1 antibody

Catalog No. ABIN6942016

The Mouse Monoclonal anti-eIF4EBP1 antibody is suitable to detect eIF4EBP1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and IF.

Quick Overview for eIF4EBP1 antibody (ABIN6942016)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: 3G1

Product Details anti-eIF4EBP1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic peptide derived from human 4E-BP1, around N-terminal.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: 4E-BP1

Background:

Synonyms: e4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, 4E BP1, 4EBP1, BP 1, BP1, e4E binding protein 1, e4E-binding protein 1, Eukaryotic translation initiation factor 4E binding protein 1, MGC4316, PHAS I, PHASI, PHAS-I, PHAS, 4E-BP1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, Phosphorylated heat and acid stable protein regulated by insulin 1, 4EBP1_HUMAN.

Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].

Gene ID: 1978

UniProt: Q13541

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling