CDK4 antibody

Catalog No. ABIN6942489

This Rabbit Monoclonal antibody specifically detects CDK4 in WB, IHC, FACS, IHC (p) and IF (cc). It exhibits reactivity toward Human and Mouse.

Quick Overview for CDK4 antibody (ABIN6942489)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 6A4

Product Details anti-CDK4 Antibody

Purpose: CDK4 Recombinant Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: C terminal Human Cdk4

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IHC-P(1:50-200), IF(ICC)(1:50-200), IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: Cdk4

Background:

Synonyms: Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

Background: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size