MYL2 antibody

Catalog No. ABIN6944202

Product Details anti-MYL2 Antibody

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MYL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified by Protein A.

Immunogen: Human Myosin Light Chain 2 between 150-250 amino acids

Clone: 10B7

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MYL2

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Alternative Name: Myosin Light Chain 2 (MYL2 Products)

Synonyms: CMH10 antibody, MLC2 antibody, 2410014J02Rik antibody, MLC-2 antibody, Mlc2 antibody, MLC-2v antibody, Mlc2v antibody, Mylpc antibody, Mlc-2 antibody, myl2 antibody, zgc:136848 antibody, XMLC2 antibody, cmh10 antibody, mlc2 antibody, G2 antibody, MLC-2A antibody, MLC2V antibody, myosin light chain 2 antibody, myosin light chain, phosphorylatable, fast skeletal muscle antibody, myosin, light polypeptide 2, regulatory, cardiac, slow antibody, myosin, light chain 2b, regulatory, cardiac, slow antibody, myosin light chain 2 S homeolog antibody, myosin, light chain 2, regulatory, cardiac, slow antibody, MYL2 antibody, Mylpf antibody, Myl2 antibody, myl2b antibody, myl2.S antibody

Background:

Synonyms: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, Cardiac myosin light chain 2, Myosin light chain 2, slow skeletal/ventricular muscle isoform, Ventricular myosin light chain 2, MLC-2, MLC-2v, MLC-2s/v, MYL2

Background: Contractile protein that plays a role in heart development and function (PubMed:10409661). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion, as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).

Gene ID: 17906

UniProt: P51667

Pathways: Myometrial Relaxation and Contraction