OGT antibody (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase))

Details for Product anti-OGT Antibody No. ABIN6944354
Antigen
  • BcDNA:GH04245
  • CG10392
  • Dmel\\CG10392
  • OGT
  • Ogt
  • P1201
  • SXC
  • Sxc
  • l(2)02637
  • l(2)NC130
  • sxc/Ogt
  • fm81g08
  • ogt
  • wu:fc12b01
  • wu:fm81g08
  • HRNT1
  • O-GLCNAC
  • 1110038P24Rik
  • 4831420N21Rik
  • AI115525
  • Ogtl
  • O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog
  • super sex combs
  • O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1
  • O-linked N-acetylglucosamine (GlcNAc) transferase
  • O-linked GlcNAc transferase
  • o-linked GlcNAc transferase
  • O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
  • ogt.L
  • sxc
  • ogt.1
  • OGT
  • ogt
  • GL50803_12081
  • GL50803_41701
  • THAPSDRAFT_264441
  • spyA
  • Ogt
Reactivity
Human, Mouse (Murine), Rat (Rattus)
97
52
44
3
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
90
8
2
Clonality (Clone)
Monoclonal ()
Conjugate
This OGT antibody is un-conjugated
8
4
4
4
4
4
4
4
3
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
64
28
27
14
12
10
4
3
3
3
2
2
2
2
1
1
Options
Immunogen Recombinant protein within Human OGT aa 1000 to the C-terminus
Clone 11F2
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others OGT products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name OGT (OGT Antibody Abstract)
Background

Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related, the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

Subcellular location: Cytoplasm, Nucleus, Mitochondrion, Cell membrane


Synonyms: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT, OGT1, HRNT1, MRX106, HINCUT-1, O-GLCNAC.
Gene ID 8473
UniProt O15294
Pathways Regulation of Carbohydrate Metabolic Process
Application Notes WB(1:500-1000), FCM(1:50-100), IHC-P(1:50-200), IF(IHC-P)(1:50-200), IF(ICC)(1:100-500)
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
Did you look for something else?