PDGFRA antibody

Catalog No. ABIN6944466

This Rabbit Monoclonal antibody specifically detects PDGFRA in WB, FACS, IHC (p), IF (p) and IF (cc). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDGFRA antibody (ABIN6944466)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 5A1

Product Details anti-PDGFRA Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic peptide within c-terminal human PDGFR Alpha

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: PDGFRA

Background:

Synonyms: Alpha-type platelet-derived growth factor receptor antibody,CD140 antigen-like family member A antibody,CD140a antibody,CD140a antigen antibody,MGC74795 antibody,PDGF alpha chain antibody,PDGF-R-alpha antibody,PDGFR 2 antibody,PDGFR alpha antibody,PDGFR2 antibody,PDGFRA antibody,PDGFRA/BCR fusion antibody,PGFRA_HUMAN antibody,Platelet derived growth factor receptor 2 antibody,Platelet derived growth factor receptor alpha antibody,Platelet derived growth factor receptor alpha polypeptide antibody,Platelet derived growth factor receptor antibody,Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody,RHEPDGFRA antibody

Background: Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades, the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Gene ID: 5156

UniProt: P16234

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling