PDHB antibody

Catalog No. ABIN6944471

Product Details anti-PDHB Antibody

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within C-terminal human Pyruvate Dehydrogenase E1 beta subunit

Clone: 10A1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: PDHB (PDHB Products)

Synonyms: CG 11876 antibody, Dmel\\CG11876 antibody, E1beta antibody, PDHB antibody, zgc:64062 antibody, wu:fc76a05 antibody, PDHBD antibody, PDHE1-B antibody, PHE1B antibody, 2610103L06Rik antibody, AL024199 antibody, C81408 antibody, CG11876 gene product from transcript CG11876-RA antibody, pyruvate dehydrogenase E1 beta subunit antibody, pyruvate dehydrogenase (lipoamide) beta antibody, CG11876 antibody, pdhb antibody, PDHB antibody, Pdhb antibody

Background:

Synonyms: Pyruvate dehydrogenase E1 component subunit beta, mitochondrial, PDHE1-B, PDHB, PHE1B, PDHBD, PDHE1B.

Background: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency). PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).

Gene ID: 5162

UniProt: P11177

Pathways: Warburg Effect