RAS antibody

Catalog No. ABIN6944747

Product Details anti-RAS Antibody

Target: RAS

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAS antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified by Protein A.

Immunogen: Human Ras aa 1-100

Clone: 11F2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RAS

Target: RAS

Abstract: RAS Products

Synonyms: asr antibody, resistance to audiogenic seizures antibody, ras antibody

Background:

Synonyms: GTPase Nras, GTPase Hras, GTPase Kras, NRAS, HRAS, KRAS

Background: Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30 % of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras.

UniProt: P01111, P01112, P01116