RNF20 antibody

Catalog No. ABIN6944787

Product Details anti-RNF20 Antibody

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RNF20 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant human RNF20 between 1 to 100 amino acids

Clone: 3H7

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Alternative Name: RNF20 (RNF20 Products)

Synonyms: 4833430L21Rik antibody, AW540162 antibody, C79397 antibody, mKIAA4116 antibody, BRE1 antibody, BRE1A antibody, hBRE1 antibody, ring finger protein 20 antibody, RNF20 antibody, Rnf20 antibody

Background:

Synonyms: E3 ubiquitin-protein ligase BRE1A, RING finger protein 20, BRE1-A, hBRE1, BRE1A

Background: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B, reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Gene ID: 56254

UniProt: Q5VTR2