Versican antibody

Catalog No. ABIN6945282

This Rabbit Monoclonal antibody specifically detects Versican in WB and IHC (p). It exhibits reactivity toward Mouse and Human.

Quick Overview for Versican antibody (ABIN6945282)

Target: Versican (Vcan)

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Versican antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2G2

Product Details anti-Versican Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human Versican aa 3100-3300

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Versican

Target: Versican (Vcan)

Alternative Name: Versican

Background:

Synonyms: Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, PG-M, Chondroitin sulfate proteoglycan 2, GHAP, VCAN, CSPG2.

Background: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a lectin-like sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length and degree of modification by glycosaminoglycan chains. Accumulation around smooth muscle cells in lesions of atherosclerosis, suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may facilitate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels. The human versican gene maps to chromosome 5q14.3.

Gene ID: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process