Recombinant GFP antibody (Alexa Fluor 647)

Catalog No. ABIN6953230

The Alpaca Monoclonal anti-GFP antibody has been validated for IF, ICC and FACS. It is suitable to detect GFP in samples from Aequorea victoria.

Quick Overview for Recombinant GFP antibody (Alexa Fluor 647) (ABIN6953230)

Target: GFP (Green Fluorescent Protein (GFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Aequorea victoria

Host: Alpaca

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Alexa Fluor 647

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1H1

Product Details anti-GFP Antibody (Alexa Fluor 647)

Expression System: E.coli

Purpose: Camelid sdAb anti-GFP conjugated with Alexa647, Clones 1H1

Specificity: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Cross-Reactivity (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Application Details

Application Notes: Recommended dilution 1:500

Comment:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Concentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP