CD3 antibody
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- Target See all CD3 Antibodies
- CD3
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD3 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunofluorescence (IF), Western Blotting (WB)
- Cross-Reactivity
- Chimpanzee
- Purification
- This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.
- Endotoxin Level
- Low endotoxin: < 0.01 EU/ug
- Clone
- UCHT1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD3 Primary Antibody
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- Application Notes
- This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.
- Comment
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2 mg/mL
- Restrictions
- For Research Use only
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- 2-8°C
- Expiry Date
- 12 months
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- Target
- CD3
- Alternative Name
- CD3 (CD3 Products)
- Synonyms
- CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody
- Background
- The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell ac- tivation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by flow cytometry - however the antibody is reported to be unsuitable for induc- tion of T cell activation in this species (Bibollet-Ruche et al. 2009. J. Virol. 82: 10271-10278).
- Gene ID
- 915, 916, 917
- UniProt
- P20963, A8MVP6, P07766, P09693
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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