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MHC II (I-A/I-E) antibody

Reactivity: Mouse FACS, IP, IF, WB, IHC (fro), IEM Host: Rat Monoclonal M5-114-15-2 unconjugated
Catalog No. ABIN6961761
  • Target See all MHC II (I-A/I-E) products
    MHC II (I-A/I-E)
    Reactivity
    Mouse
    Host
    • 23
    Rat
    Clonality
    • 23
    Monoclonal
    Conjugate
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MHC II (I-A/I-E) antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)
    Purification
    This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.
    Clone
    M5-114-15-2
    Isotype
    IgG2b kappa
  • Application Notes
    This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.
    Comment

    0.5 mg/mL

    Restrictions
    For Research Use only
  • Buffer
    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    2-8°C
    Expiry Date
    12 months
  • Target
    MHC II (I-A/I-E)
    Alternative Name
    MHC Class II (I-A/I-E) (MHC II (I-A/I-E) Products)
    Background
    The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
    Gene ID
    14961, 14969
    UniProt
    P14483
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