Interferon gamma antibody (PromoFluor-488 Premium)
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- Target See all Interferon gamma (IFNG) Antibodies
- Interferon gamma (IFNG)
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Reactivity
- Ferret
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Interferon gamma antibody is conjugated to PromoFluor-488 Premium
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Application
- Flow Cytometry (FACS)
- Specificity
- Native and recombinant IFN-γ
- Purification
- Purified from in vitro cultures by protein G affinity chromatography.
- Immunogen
- Recombinant ferret IFN-gamma
- Clone
- MTF14
- Isotype
- IgG1
- Top Product
- Discover our top product IFNG Primary Antibody
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- Application Notes
- Detection of ferret IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable ferret spleen cells. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MTF14-PF488P (right panel). The left panel depicts cells stained with a matched isotype control antibody. The dot plots derive from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- in PBS with 0.2 % BSA and 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
- Expiry Date
- 18 months
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- IFNG (IFNG Products)
- Synonyms
- IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody
- Gene ID
- 101693741
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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