Interferon gamma antibody (Biotin)

Catalog No. ABIN6963775

Product Details anti-Interferon gamma Antibody (Biotin)

Target: Interferon gamma (IFNG)

Reactivity: Cow, Sheep, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: ELISA, Flow Cytometry (FACS), ELISpot

Specificity: Native and recombinant IFN-γ

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

Characteristics: This monoclonal antibody enables specific detection of bovine IFN-γ in immunoassays such as ELISpot, FluoroSpot, ELISA, and flow cytometry. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant bovine IFN-gamma

Clone: MT307

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963775, ABIN7448244, ABIN6963776 and ABIN6963777 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb .1 . ABIN6963775, ABIN7448244, ABIN6963776 and ABIN6963777 is also recommended for use in flow cytometry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-Interferon gamma antibody (Biotin) (ABIN6963775)

Sajiki, Konnai, Okagawa, Nishimori, Maekawa, Goto, Ikebuchi, Nagata, Kawaji, Kagawa, Yamada, Kato, Nakajima, Suzuki, Murata, Mori, Ohashi: "Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease." in: Infection and immunity, Vol. 86, Issue 5, (2018) (PubMed).

Orbegozo-Medina, Martínez-Sernández, González-Warleta, Castro-Hermida, Mezo, Ubeira: "Vaccination of sheep with Quil-A® adjuvant expands the antibody repertoire to the Fasciola MF6p/FhHDM-1 antigen and administered together impair the growth and antigen release of flukes." in: Vaccine, Vol. 36, Issue 15, pp. 1949-1957, (2018) (PubMed).

Rojas, Moreno, Valcárcel, Peña, Sevilla, Martín et al.: "Vaccination with recombinant adenoviruses expressing the peste des petits ruminants virus F or H proteins overcomes viral immunosuppression and induces protective immunity against PPRV challenge in ..." in: PLoS ONE, Vol. 9, Issue 7, pp. e101226, (2015) (PubMed).

Vordermeier, Dean, Rosenkrands, Agger, Andersen, Kaveh, Hewinson, Hogarth: "Adjuvants induce distinct immunological phenotypes in a bovine tuberculosis vaccine model." in: Clinical and vaccine immunology : CVI, Vol. 16, Issue 10, pp. 1443-8, (2009) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG (IFNG Products)

Synonyms: IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody

Gene ID: 281237, 443396

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy