IL-2 antibody (Biotin)

Catalog No. ABIN6963854

This Rat Monoclonal antibody specifically detects IL-2 in ELISA and ELISpot. It exhibits reactivity toward Mouse. It has been mentioned in 4+ publications

Quick Overview for IL-2 antibody (Biotin) (ABIN6963854)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is conjugated to Biotin

Application: ELISA, ELISpot

Clone: 5H4

Product Details anti-IL-2 Antibody (Biotin)

Specificity: Native and recombinant mouse IL-2

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant mouse IL-2

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-IL-2 antibody (Biotin) (ABIN6963854)

Suschak, Bagley, Shoemaker, Six, Kwilas, Dupuy, Schmaljohn: "The Genetic Adjuvants Interleukin-12 and Granulocyte-Macrophage Colony Stimulating Factor Enhance the Immunogenicity of an Ebola Virus Deoxyribonucleic Acid Vaccine in Mice." in: The Journal of infectious diseases, Vol. 218, Issue suppl_5, pp. S519-S527, (2019) (PubMed).

Mazor, Crown, Addissie, Jang, Kaplan, Pastan: "Elimination of murine and human T-cell epitopes in recombinant immunotoxin eliminates neutralizing and anti-drug antibodies in vivo." in: Cellular & molecular immunology, Vol. 14, Issue 5, pp. 432-442, (2018) (PubMed).

Ashrafi, Shapouri, Shirkhani, Mahdavi: "Anti-tumor effects of propranolol: Adjuvant activity on a transplanted murine breast cancer model." in: Biomedicine & pharmacotherapy, Vol. 104, pp. 45-51, (2018) (PubMed).

Yazdi, Varastehmoradi, Faghfuri, Mavandadnejad, Mahdavi, Shahverdi: "Adjuvant Effect of Biogenic Selenium Nanoparticles Improves the Immune Responses and Survival of Mice Receiving 4T1 Cell Antigens as Vaccine in Breast Cancer Murine Model." in: Journal of nanoscience and nanotechnology, Vol. 15, Issue 12, pp. 10165-72, (2017) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Gene ID: 16183

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation