TNF alpha antibody (PromoFluor-488 Premium)

Catalog No. ABIN6963901

The PromoFluor-488 Premium-conjugated Mouse Monoclonal anti-TNF alpha antibody (Clone MT15B15) (ABIN6963901) specifically detects TNF alpha in FACS. The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (PromoFluor-488 Premium) (ABIN6963901)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to PromoFluor-488 Premium

Application: Flow Cytometry (FACS)

Clone: MT15B15

Product Details anti-TNF alpha Antibody (PromoFluor-488 Premium)

Specificity: Native and recombinant TNF-α

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human TNF-alpha

Isotype: IgG1

Application Details

Application Notes: Detection of human TNF-α producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of TNF-α by flow cytometry in viable human PBMC. Cells were stimulated for 3 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with an anti-CD3-PE antibody, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with antibody MT15B15-PF488P (right panel). Matched isotype control antibody (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNFA

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome