TNF alpha antibody (Biotin)

Catalog No. ABIN6963904

Cited in 1 publication. The Biotin-conjugated Mouse Monoclonal anti-TNF alpha antibody (Clone MT15B15) (ABIN6963904) specifically detects TNF alpha in WB, ELISA, FACS and ELISpot. The antibody is reactive with Non-Human Primate samples.

Quick Overview for TNF alpha antibody (Biotin) (ABIN6963904)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), ELISpot

Clone: MT15B15

Product Details anti-TNF alpha Antibody (Biotin)

Specificity: Native and recombinant TNF-α

Cross-Reactivity (Details): The monoclonal antibody reacts with human TNF-α and cross-reacts with TNF-α from non-human primates.

Characteristics: This monoclonal antibody enables specific detection of monkey TNF-α in immunoassays such as ELISpot, FluoroSpot, ELISA, flow cytometry, and Western blot. Serum/Plasma samples

Purification: Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human TNF-alpha

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

ABIN6963898, ABIN6963901, ABIN6963902, ABIN6963904, ABIN7489162, ABIN6963905 and ABIN6963906 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb ABIN6963903 and ABIN7489161 . The antibody is also suitable for Western blot and Flow cytometry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-TNF alpha antibody (Biotin) (ABIN6963904)

Phuah, Wong, Gideon, Maiello, Coleman, Hendricks, Ruden, Cirrincione, Chan, Lin, Flynn: "Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques." in: Infection and immunity, Vol. 84, Issue 5, pp. 1301-1311, (2017) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNFA

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome