Estrogen Receptor alpha antibody (C-Term)

Catalog No. ABIN6971631

The Mouse Monoclonal anti-Estrogen Receptor alpha antibody is suitable to detect Estrogen Receptor alpha in samples from Human. It has been validated for WB, IP and ChIP.

Quick Overview for Estrogen Receptor alpha antibody (C-Term) (ABIN6971631)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: F3

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: C-Term

Characteristics: ER-a (estrogen receptor a) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ER-a localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis. Estrogen Receptor alpha antibody (mAb) (Clone F3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogen: This ER-alpha antibody was raised against a peptide within the C-terminal region of human Estrogen Receptor alpha.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor α

Molecular Weight: 66 kDa

NCBI Accession: NP_000116

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly