Histone H1 antibody (AA 109-256)

Catalog No. ABIN6971739

Product Details anti-Histone H1 Antibody

Target: Histone H1

Binding Specificity: AA 109-256

Reactivity: Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Characteristics: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression. Histone H1 antibody (mAb) (Clone 1G6-E3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Drosophila samples.

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This region of the protein is not conserved in mammals, the antibody is not likely to cross-react to human, mouse or rat Histone H1. 

Clone: 1G6-E3

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H1

Target: Histone H1

Abstract: Histone H1 Products

Synonyms: H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody

Molecular Weight: 35 kDa

NCBI Accession: NP_001027286