Histone H4 antibody (2meArg3 (symetric))

Catalog No. ABIN6972145

Product Details anti-Histone H4 Antibody

Target: Histone H4

Binding Specificity: 2meArg3 (symetric)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Characteristics: Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Symmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional repression. Histone H4 dimethyl Arg3 symmetric methylation (H4R2me2s) is catalyzed by PRMT5 and PRMT7. Histone H4R3me2s (symmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogen: This Histone H4 dimethyl Arg3 (H3R3me2s) antibody was raised against a peptide containing dimethyl Arg3 (symmetric) of human Histone H4.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H4

Target: Histone H4

Abstract: Histone H4 Products

Synonyms: hist1h4l antibody, H1ft antibody, Hist4 antibody, Hist4h4 antibody, H4 antibody, X04652 antibody, FO108 antibody, H4/n antibody, H4F2 antibody, H4FN antibody, HIST2H4 antibody, H4C13 antibody, H4C7 antibody, F8N16.2 antibody, F8N16_2 antibody, histone H4 antibody, BcDNA:RH52884 antibody, CG3379 antibody, Dmel\CG3379 antibody, H4r antibody, His4-88CD antibody, His4R antibody, histone cluster 1, H4a L homeolog antibody, histone cluster 1 H4 family member B antibody, histone cluster 2, H4 antibody, histone cluster 2 H4 family member a antibody, histone H4 antibody, Histone H4 replacement antibody, Histone H4 antibody, hist1h4a.L antibody, Hist1h4b antibody, Hist2h4 antibody, HIST2H4A antibody, H4 antibody, H4C14 antibody, HIS4 antibody, HIST2H4 antibody, His4r antibody, his-38 antibody

Molecular Weight: 10 kDa

NCBI Accession: NP_778224