MED15 antibody (C-Term)

Catalog No. ABIN6972333

The Rabbit Polyclonal anti-MED15 antibody has been validated for WB and IP. It is suitable to detect MED15 in samples from Human.

Quick Overview for MED15 antibody (C-Term) (ABIN6972333)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-MED15 Antibody

Binding Specificity: C-Term

Characteristics: MED15 (mediator of RNA polymerase II transcription subunit 15) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. MED15 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of human MED15.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15

Molecular Weight: 100 kDa

NCBI Accession: NP_001003891

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha