MGMT antibody (C-Term)

Catalog No. ABIN6972348

The Rabbit Polyclonal anti-MGMT antibody is suitable to detect MGMT in samples from Human. It has been validated for WB and IP.

Quick Overview for MGMT antibody (C-Term) (ABIN6972348)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-MGMT Antibody

Binding Specificity: C-Term

Characteristics: MGMT (O-6 methylguanine DNA methyltransferase) is an enzyme involved in DNA damage repair. MGMT transfers methyl groups from O-6 methylguanine methylated DNA, and other methylated adducts, to a cysteine residue in itself, thus reversing DNA damage caused by alkylation. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT helps prevent DNA alkylation events from causing mutations. It also contributes to resistance to chemotherapy in cancer patients being treated with alkylating drugs. MGMT antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogen: This MGMT antibody was raised against a peptide derived from the C-terminal region of human MGMT.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Molecular Weight: 28 kDa

NCBI Accession: NP_002403

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus