DnaK antibody

Catalog No. ABIN6976323

Product Details

Target: DnaK

Reactivity: Mycobacteria

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC)

Specificity: Mycobacterial HSP70 and cross-reactive with E. coli DnaK

Cross-Reactivity (Details): cross reactive with E.coli DNAK

Purification: Protein G affinity purified

Purity: > 95 %

Immunogen: HSP70 protein from Mycobacterium avium ssp. paratuberculosis (MAP)

Clone: 7D9D7

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: Store at 4 °C, short term (1-2 weeks). For long-term storage, aliquot and keep at or below -20° C. Avoid repeated freeze-thaw cycles

Target Details

Target: DnaK

Background: Mycobaterial HSP70 is the prokaryotic analogue of eukaryotic Hsp70 and is an effective subunit vaccine against bovine paratuberculosis (Koets et al., 2006). HSP70 belongs to a group of heat shock proteins that assist in the assembly, folding and translocation of other proteins