mCherry antibody (AA 1-236) (FITC)

Catalog No. ABIN6987372

Product Details anti-mCherry Antibody (FITC)

Target: mCherry (mCherry Fluorescent Protein)

Binding Specificity: AA 1-236

Reactivity: Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This mCherry antibody is conjugated to FITC

Application: Western Blotting (WB)

Cross-Reactivity: Tag

Cross-Reactivity (Details): mCherry-Tag

Purification: Purified by Protein A.

Immunogen: Recombinant mCherry protein

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for mCherry

Target: mCherry (mCherry Fluorescent Protein)

Alternative Name: mCherry-Tag (mCherry Products)

Background:

Synonyms: mCherry Tag, monomeric cherry red fluorescent protein.

Background: mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.