P4HB antibody

Catalog No. ABIN6989700

Product Details anti-P4HB Antibody

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic peptide within C-terminal Human P4HB.

Clone: 9H8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: P4HB (P4HB Products)

Synonyms: DSI antibody, ERBA2L antibody, GIT antibody, P4Hbeta antibody, PDI antibody, PDIA1 antibody, PHDB antibody, PO4DB antibody, PO4HB antibody, PROHB antibody, PDA2 antibody, PDIP antibody, PDIR antibody, ERp59 antibody, Pdia1 antibody, Thbp antibody, p55 antibody, XPDIp antibody, pdi antibody, 1810041F13Rik antibody, AI661267 antibody, Pdip antibody, Pdipl antibody, p58 antibody, prolyl 4-hydroxylase subunit beta antibody, protein disulfide isomerase family A member 2 antibody, prolyl 4-hydroxylase, beta polypeptide antibody, protein disulfide isomerase family A member 2 L homeolog antibody, protein disulfide isomerase associated 2 antibody, prolyl 4-hydroxylase subunit beta L homeolog antibody, protein disulfide-isomerase antibody, P4HB antibody, PDIA2 antibody, P4hb antibody, pdia2.L antibody, Pdia2 antibody, p4hb.L antibody, LOC8281065 antibody

Background:

Synonyms: Cellular thyroid hormone binding protein antibody, Cellular thyroid hormone-binding protein antibody, Collagen prolyl 4 hydroxylase beta antibody, Disulphide Isomerase antibody, DSI antibody, EC 5.3.4.1 antibody, Endoplasmic reticulum resident protein 59 antibody, ER protein 59 antibody, ERBA2L antibody, ERp59 antibody, GIT antibody, Gltathione insulin transhydrogenase antibody, Glutathione insulin transhydrogenase antibody, P4HB antibody, P4Hbeta antibody, p55 antibody, PDI antibody, PDIA1 antibody, PDIA1_HUMAN antibody, PDIR antibody, PHDB antibody, PO4DB antibody, PO4HB antibody, Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody,

Background: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Gene ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism