ROCK1 antibody

Catalog No. ABIN6989875

This Rabbit Polyclonal antibody specifically detects ROCK1 in WB, IHC, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ROCK1 antibody (ABIN6989875)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ROCK1 Antibody

Purpose: ROCK1 Polyclonal Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400), IP(1-2 μg), IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background:

Synonyms: ROCK1,P160ROCK,ROCK-I

Background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.

Gene ID: 6093

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling