GSK3 alpha/beta antibody (pTyr216, pTyr279)
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- Target See all GSK3 alpha/beta (GSK3a/b) products
- GSK3 alpha/beta (GSK3a/b) (Glycogen Synthase Kinase 3 alpha/beta (GSK3a/b))
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Binding Specificity
- pTyr216, pTyr279
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This GSK3 alpha/beta antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunoprecipitation (IP)
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified by Protein A.
- Immunogen
- Synthetic phospho-Peptide corresponding to residues surrounding Tyr216 and 279 of human GSK3(alpha+beta).
- Clone
- 5G8
- Isotype
- IgG
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- Application Notes
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WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IP(1-2 μg)
IHC() - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- GSK3 alpha/beta (GSK3a/b) (Glycogen Synthase Kinase 3 alpha/beta (GSK3a/b))
- Alternative Name
- GSK3(alpha+beta) (GSK3a/b Products)
- Background
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Synonyms: Factor A antibody, Glycogen synthase kinase 3 alpha antibody, Glycogen synthase kinase 3 beta antibody, GSK3 alpha antibody, GSK3 beta antibody, GSK3B antibody
Background: Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
- Gene ID
- 2932
- UniProt
- P49840
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