Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Purification
BAFF Antibody is Ion exchange chromatography purified.
Immunogen
Anti-BAFF antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF. The immunogen is located within the last 50 amino acids of BAFF.
TNFSF13B
Reactivity: Human
WB, ELISA, FACS, ICC, IF
Host: Mouse
Monoclonal
H4-C7
unconjugated
Application Notes
WB: 0.25-1 μ,g/mL, ICC: 1 μ,g/mL, IF: 20 μ,g/mL.
Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples, Immunofluorescence in human samples, Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
BAFF Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
BAFF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
BAFF Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κ,B and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.