Caspase 12 antibody (AA 90-140)

Catalog No. ABIN6990350

Product Details anti-Caspase 12 Antibody

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Binding Specificity: AA 90-140

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: Caspase-12 Antibody is affinity chromatography purified via peptide column.

Immunogen: Caspase-12 antibody was raised against a 17 amino acid peptide near the center of murine Caspase-12. The immunogen is located within amino acids 90 - 140 of Caspase-12.

Isotype: IgG

Application Details

Application Notes: Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Caspase-12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: Caspase-12 (CASP12 Products)

Synonyms: Caspase-12 antibody, CASP-12 antibody, CASP12P1 antibody, caspase 12 antibody, caspase 12 (gene/pseudogene) antibody, Casp12 antibody, CASP12 antibody

Background: Caspase-12 Antibody: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta.

Molecular Weight: Predicted: 46 kDa

Observed: 55 kDa

Gene ID: 12364

UniProt: O08736

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response