IKAP/p150 antibody (C-Term)

Catalog No. ABIN6990353

Product Details anti-IKAP/p150 Antibody

Target: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKAP/p150 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.

Purification: IKAP Antibody is affinity chromatography purified via peptide column.

Immunogen: IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP. The immunogen is located within the last 50 amino acids of IKAP.

Isotype: IgG

Application Details

Application Notes: IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IKAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IKAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IKAP/p150

Target: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Alternative Name: IKAP (ELP1 Products)

Synonyms: DYS antibody, ELP1 antibody, FD antibody, IKAP antibody, IKI3 antibody, TOT1 antibody, IKBKAP antibody, DKFZp469N1415 antibody, 3110040G09Rik antibody, 6030413P05 antibody, C78473 antibody, Elp1 antibody, Ikbkap antibody, elongator complex protein 1 antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein antibody, ielongator complex protein 1 S homeolog antibody, inhibitor of kappa light polypeptide enhancer in B cells, kinase complex-associated protein antibody, ELP1 antibody, Elp1 antibody, IKBKAP antibody, elp1.S antibody, elp1 antibody, Ikbkap antibody

Background: IKAP Antibody: IKAP was initially identified as a scaffold protein of the Iκ,B kinase complex that could bind to IKKα, IKKβ, NF-κ,B, and the NF-κ,B-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.

Molecular Weight: Predicted: 134, 147 kDa

Observed: 105, 145 kDa

Gene ID: 8518

UniProt: O95163