XIAP antibody (C-Term)

Catalog No. ABIN6990469

Product Details anti-XIAP Antibody

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Binding Specificity: AA 420-470, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XIAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: XIAP Antibody is affinity chromatography purified via peptide column.

Immunogen: XIAP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP. The immunogen is located within amino acids 420 - 470 of XIAP.

Isotype: IgG

Application Details

Application Notes: XIAP antibody can be used for the detection of XIAP by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: XIAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: XIAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: XIAP (XIAP Products)

Synonyms: API3 antibody, BIRC4 antibody, IAP-3 antibody, ILP1 antibody, MIHA antibody, XLP2 antibody, hIAP-3 antibody, hIAP3 antibody, api3 antibody, birc4 antibody, ilp1 antibody, miha antibody, xlp2 antibody, xxiap antibody, 1110015C02Rik antibody, Aipa antibody, Api3 antibody, Birc4 antibody, IAP3 antibody, ILP-1 antibody, mXIAP antibody, riap3 antibody, fb73d01 antibody, wu:fb73d01 antibody, zgc:91873 antibody, BIRC8 antibody, X-linked inhibitor of apoptosis antibody, X-linked inhibitor of apoptosis L homeolog antibody, XIAP antibody, xiap antibody, xiap.L antibody, Xiap antibody

Background: XIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The X-chromosome linked inhibitor of apoptosis (XIAP) contains 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7, and -9. Upregulation of XIAP expression can protect cells from apoptosis induced by low level radiation, conversely, decreased XIAP expression by antisense targeting resulted in increased cell death following low level radiation. Two negative regulators, termed XAF-1 and Smac, can bind and inhibit XIAP activity.

Molecular Weight: Predicted: 55 kDa

Observed: 53 kDa

Gene ID: 331

NCBI Accession: NP_001158

UniProt: P98170

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis