Bcl-2 antibody (AA 80-130)

Catalog No. ABIN6990471

The Rabbit Polyclonal anti-Bcl-2 antibody (ABIN6990471) specifically detects Bcl-2 in WB, ELISA and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for Bcl-2 antibody (AA 80-130) (ABIN6990471)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 80-130

Purification: Bcl-2 Antibody is Ion exchange chromatography purified.

Immunogen: Anti-Bcl-2 antibody was raised against a peptide corresponding to 14 amino acids near the center of human Bcl-2. The immunogen is located within amino acids 80-130 of Bcl-2.

Isotype: IgG

Application Details

Application Notes: WB: 1-4μ,g/mL, ICC: 2 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Bcl-2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Bcl-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background: Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Molecular Weight: Predicted: 26kD

Observed: 26 kD kDa

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling