Caspase 8 antibody (C-Term)

Catalog No. ABIN6990524

The Rabbit Polyclonal anti-Caspase 8 antibody has been validated for WB, ELISA, ICC, IF and IHC (p). It is suitable to detect Caspase 8 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 8 antibody (C-Term) (ABIN6990524)

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 8 Antibody

Binding Specificity: C-Term

Specificity: Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

Purification: Caspase-8 Antibody is affinity chromatography purified via peptide column.

Immunogen: Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. The immunogen is located within the last 50 amino acids of Caspase-8.

Isotype: IgG

Application Details

Application Notes: Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL and immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Caspase-8 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Caspase-8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8

Background: Caspase-8 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain, subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.

Molecular Weight: 55 kDa

Gene ID: 841

NCBI Accession: NP_001219

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades