HRK antibody

Catalog No. ABIN6990622

Product Details anti-HRK Antibody

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRK antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Hrk Antibody is affinity chromatography purified via peptide column.

Immunogen: HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk. The immunogen is located within the last 50 amino acids of Hrk.

Isotype: IgG

Application Details

Application Notes: Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Hrk Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Hrk antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for HRK

Target: HRK (Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))

Alternative Name: Hrk (HRK Products)

Synonyms: DP5 antibody, HARAKIRI antibody, AI838259 antibody, Bid3 antibody, harakiri antibody, Dp5 antibody, harakiri, BCL2 interacting protein antibody, harakiri, BCL2 interacting protein (contains only BH3 domain) antibody, HRK antibody, Hrk antibody

Background: Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.

Molecular Weight: Predicted: 10 kDa

Observed: 12 kDa

Gene ID: 8739

NCBI Accession: NP_003797

UniProt: O00198