BIF (C-Term) antibody
Reactivity: Human, Mouse
ICC, ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- BIF
- Binding Specificity
- C-Term
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
- Purification
- Bif Antibody is affinity chromatography purified via peptide column.
- Immunogen
- BIF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BIF. The immunogen is located within the last 50 amino acids of Bif.
- Isotype
- IgG
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- Application Notes
-
BIF antibody can be used for the detection of BIF by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Bif Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- Bif antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
- Target
- BIF
- Background
- Bif Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.
- Gene ID
- 51100
- UniProt
- Q9Y371
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