BIK antibody (N-Term)

Catalog No. ABIN6990642

Product Details anti-BIK Antibody

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: Bik Antibody is affinity chromatography purified via peptide column.

Immunogen: BIK antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human BIK. The immunogen is located within the first 50 amino acids of Bik.

Isotype: IgG

Application Details

Application Notes: BIK antibody can be used for the detection of BIK by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Bik Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Bik antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BIK

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Alternative Name: Bik (BIK Products)

Synonyms: BIK antibody, Biklk antibody, Blk antibody, Nbk antibody, BIP1 antibody, BP4 antibody, NBK antibody, si:dkey-31f5.6 antibody, BCL2 interacting killer antibody, BCL2-interacting killer antibody, BCL2-interacting killer (apoptosis-inducing) antibody, BIK antibody, Bik antibody, bik antibody

Background: Bik Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.

Molecular Weight: Predicted: 18 kDa

Observed: 22 kDa

Gene ID: 638

UniProt: Q13323

Pathways: Positive Regulation of Endopeptidase Activity