PAFAH1B1 antibody (C-Term)

Catalog No. ABIN6990777

Product Details anti-PAFAH1B1 Antibody

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Binding Specificity: AA 340-390, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAFAH1B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: LIS1 Antibody is affinity chromatography purified via peptide column.

Immunogen: LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1. The immunogen is located within amino acids 340 - 390 of LIS1.

Isotype: IgG

Application Details

Application Notes: LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LIS1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LIS1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for PAFAH1B1

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Alternative Name: LIS1 (PAFAH1B1 Products)

Synonyms: LIS1 antibody, LIS2 antibody, MDCR antibody, MDS antibody, PAFAH antibody, LIS-1 antibody, Lis1 antibody, MMS10-U antibody, Mdsh antibody, Ms10u antibody, Pafaha antibody, lis2 antibody, mdcr antibody, mds antibody, pafah antibody, pafah1b1-b antibody, PAFAHA antibody, Lis1a antibody, platelet activating factor acetylhydrolase 1b regulatory subunit 1 antibody, platelet-activating factor acetylhydrolase, isoform 1b, subunit 1 antibody, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 antibody, platelet activating factor acetylhydrolase 1b regulatory subunit 1 L homeolog antibody, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1a antibody, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1b antibody, PAFAH1B1 antibody, Pafah1b1 antibody, pafah1b1.L antibody, pafah1b1a antibody, pafah1b1b antibody

Background: LIS1 Antibody: Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3epsilon which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.

Gene ID: 5048

UniProt: P43034

Pathways: M Phase, Regulation of Cell Size