Beclin 1 antibody (N-Term)

Catalog No. ABIN6990803

Product Details anti-Beclin 1 Antibody

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Binding Specificity: AA 40-90, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purification: Beclin-1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Beclin-1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1. The immunogen is located within amino acids 40 - 90 of Beclin-1.

Isotype: IgG

Application Details

Application Notes: Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Beclin-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Beclin-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1 (BECN1 Products)

Synonyms: beclin-1 antibody, atg6 antibody, vps30 antibody, beclin1 antibody, Beclin-1 antibody, becn1 antibody, zgc:73097 antibody, ATG6 antibody, VPS30 antibody, 4921513J16Rik antibody, 5430417M23Rik antibody, Atg6 antibody, beclin 1 antibody, beclin 1, autophagy related antibody, beclin 1 protein antibody, beclin-1 antibody, beclin family protein antibody, beclin 1, autophagy related L homeolog antibody, BECN1 antibody, becn1 antibody, atg6 antibody, CpipJ_CPIJ011087 antibody, PTRG_07825 antibody, SJAG_01876 antibody, Becn1 antibody, becn1.L antibody

Background: Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy