GPR124 antibody (AA 560-610)

Catalog No. ABIN6990814

Product Details anti-GPR124 Antibody

Target: GPR124 (G Protein-Coupled Receptor 124 (GPR124))

Binding Specificity: AA 560-610

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR124 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: TEM5 Antibody is affinity chromatography purified via peptide column.

Immunogen: TEM5 antibody was raised against a 14 amino acid synthetic peptide near the center of human TEM5. The immunogen is located within amino acids 560 - 610 of TEM5.

Isotype: IgG

Application Details

Application Notes: TEM5 antibody can be used for detection of TEM5 by Western blot at 2 and 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TEM5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TEM5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for GPR124

Target: GPR124 (G Protein-Coupled Receptor 124 (GPR124))

Alternative Name: TEM5 (GPR124 Products)

Synonyms: TEM5 antibody, 8430414O08Rik antibody, 9530074E10Rik antibody, Tem5 antibody, mKIAA1531 antibody, MGC69043 antibody, si:dkey-208c22.2 antibody, GPR124 antibody, adhesion G protein-coupled receptor A2 antibody, adhesion G protein-coupled receptor A2 L homeolog antibody, G-protein coupled receptor 124 antibody, ADGRA2 antibody, Adgra2 antibody, adgra2.L antibody, adgra2 antibody, LOC616111 antibody

Background: TEM5 Antibody: Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

Gene ID: 25960

UniProt: Q96PE1