FGF4 antibody (C-Term)

Catalog No. ABIN6990842

The Rabbit Polyclonal anti-FGF4 antibody is suitable to detect FGF4 in samples from Human and Mouse. It has been validated for WB, ELISA, ICC and IF.

Quick Overview for FGF4 antibody (C-Term) (ABIN6990842)

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-FGF4 Antibody

Binding Specificity: C-Term

Purification: FGF4 Antibody is affinity chromatography purified via peptide column.

Immunogen: FGF4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4. The immunogen is located within the last 50 amino acids of FGF4.

Isotype: IgG

Application Details

Application Notes: FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: FGF4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: FGF4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for FGF4

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Alternative Name: FGF4

Background: FGF4 Antibody: Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

Gene ID: 2249

UniProt: P08620

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance