WNT10B antibody (AA 130-180)

Catalog No. ABIN6990912

Product Details anti-WNT10B Antibody

Target: WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))

Binding Specificity: AA 130-180

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT10B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Two isoforms of Wnt10b are known to exist, this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.

Purification: Wnt10b Antibody is affinity chromatography purified via peptide column.

Immunogen: Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b. The immunogen is located within amino acids 130 - 180 of Wnt10b.

Isotype: IgG

Application Details

Application Notes: Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Wnt10b Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Wnt10b antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for WNT10B

Target: WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))

Alternative Name: Wnt10b (WNT10B Products)

Synonyms: WNT10B antibody, Xwnt-10b antibody, Xwnt10b antibody, wnt-12 antibody, Wnt12 antibody, SHFM6 antibody, WNT-12 antibody, Xwnt-10 antibody, wnt10 antibody, wnt10-A antibody, Zwnt[d] antibody, wnt10br antibody, wnt[d] antibody, Wnt family member 10B antibody, wingless-type MMTV integration site family, member 10B antibody, Wnt family member 10B S homeolog antibody, wingless-type MMTV integration site family, member 10b antibody, WNT10B antibody, wnt10b antibody, Wnt10b antibody, wnt10b.S antibody

Background: Wnt10b Antibody: Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.

Molecular Weight: Predicted: 43 kDa

Observed: 47 kDa

Gene ID: 7480

NCBI Accession: NP_003385

UniProt: O00744

Pathways: WNT Signaling, Skeletal Muscle Fiber Development