LASS5 antibody (N-Term)

Catalog No. ABIN6990941

Product Details anti-LASS5 Antibody

Target: LASS5 (LAG1 Homolog, Ceramide Synthase 5 (LASS5))

Binding Specificity: AA 90-140, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LASS5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6.

Purification: LASS5 Antibody is affinity chromatography purified via peptide column.

Immunogen: LASS5 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human LASS5. The immunogen is located within amino acids 90 - 140 of LASS5.

Isotype: IgG

Application Details

Application Notes: LASS5 antibody can be used for detection of LASS5 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LASS5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: LASS5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for LASS5

Target: LASS5 (LAG1 Homolog, Ceramide Synthase 5 (LASS5))

Alternative Name: LASS5 (LASS5 Products)

Synonyms: lass5 antibody, trh4 antibody, LASS5 antibody, Trh4 antibody, 2310081H14Rik antibody, AU045339 antibody, AW544927 antibody, Lass5 antibody, ceramide synthase 5 antibody, ceramide synthase 5 S homeolog antibody, CERS5 antibody, cers5 antibody, cers5.S antibody, Cers5 antibody

Background: LASS5 Antibody: The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.

Gene ID: 91012

NCBI Accession: NP_671723

UniProt: Q8N5B7