Western Blotting (WB), ELISA, Immunofluorescence (IF)
Purification
EFHD1 Antibody is affinity chromatography purified via peptide column.
Immunogen
EFHD1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1. The immunogen is located within the first 50 amino acids of EFHD1.
EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 μ,g/mL. Antibody can also be used for immunoflourescence starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
EFHD1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
EFHD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
MGC76180 antibody, MGC80733 antibody, 4931430I01Rik antibody, AI452351 antibody, PP3051 antibody, mitocalcin antibody, MST133 antibody, MSTP133 antibody, SWS2 antibody, RGD1559565 antibody, EF-hand domain family member D1 antibody, EF-hand domain family member D1 L homeolog antibody, EF-hand domain family, member D1 antibody, EF hand domain containing 1 antibody, efhd1 antibody, EFHD1 antibody, efhd1.L antibody, Efhd1 antibody
Background
EFHD1 Antibody: EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.