Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
APC3 Antibody is affinity chromatography purified via peptide column.
Immunogen
APC3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human APC3. The immunogen is located within the last 50 amino acids of APC3.
APC3 antibody can be used for detection of APC3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
APC3 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
APC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
CDC27
(Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
APC3 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.