alpha Fetoprotein antibody (AA 340-390)

Catalog No. ABIN6991386

Product Details anti-alpha Fetoprotein Antibody

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Binding Specificity: AA 340-390

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: AFP Antibody is affinity chromatography purified via peptide column.

Immunogen: AFP antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP. The immunogen is located within amino acids 340 - 390 of AFP.

Isotype: IgG

Application Details

Application Notes: AFP antibody can be used for detection of AFP by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: AFP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: AFP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP (AFP Products)

Synonyms: FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody

Background: AFP Antibody: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found in individuals with no obvious pathology. AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. AFP levels in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Gene ID: 174

NCBI Accession: NP_001125

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process