At least three isoforms of ZBTB3 are known to exist. This antibody is predicted to not cross-react with other ZBTB protein family members.
Purification
ZBTB3 Antibody is affinity chromatography purified via peptide column.
Immunogen
ZBTB3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB3. The immunogen is located within amino acids 510 - 560 of ZBTB3.
ZBTB3
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Cy5.5
Application Notes
ZBTB3 antibody can be used for detection of ZBTB3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ZBTB3 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
ZBTB3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
ZBTB3
(Zinc Finger and BTB Domain Containing 3 (ZBTB3))
ZBTB3 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. While little is known about ZBTB3, the related protein ZBTB2 is thought to be phosphorylated in response to the DNA damage, probably by either ATM or ATR. Other ZBTB proteins, such as ZBTB4 and ZBTB38 bind methylated DNA and repress transcription, suggesting that ZBTB3 may also act as a transcription repressor.