Three isoforms of LRRFIP2 are known to exist, this antibody will recognize all three. LRRFIP2 antibody is predicted to not cross-react with other LRRFIP family members.
Purification
LRRFIP2 Antibody is affinity chromatography purified via peptide column.
Immunogen
LRRFIP2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human LRRFIP2. The immunogen is located within amino acids 620 - 670 of LRRFIP2.
LRRFIP2
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
LRRFIP2 antibody can be used for detection of LRRFIP2 by Western blot at 0.5 and 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
LRRFIP2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
LRRFIP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
LRRFIP2
(Leucine Rich Repeat (In FLII) Interacting Protein 2 (LRRFIP2))
HUFI-2 antibody, 5133400F20Rik antibody, AI850587 antibody, wu:fj34c02 antibody, zgc:113956 antibody, zgc:63771 antibody, LRR binding FLII interacting protein 2 antibody, leucine rich repeat (in FLII) interacting protein 2 antibody, leucine rich repeat (in FLII) interacting protein 2 L homeolog antibody, LRRFIP2 antibody, Lrrfip2 antibody, lrrfip2 antibody, lrrfip2.L antibody
Background
LRRFIP2 Antibody: The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI. It can activate the Wnt signaling pathway in cultured cells and is thought to be a component of the Wnt signaling pathway that modulates Wnt signaling through interactions with Disheveled to increase the cellular levels and transcription activity of beta-catenin. LRRFIP2 has recently been characterized as a positive regulator of the TLR4 signaling pathway for activating NF-κ,B during the early host response to LPS stimulation through binding to the TLR adaptor protein MyD88, and that this interaction with MyD88 is governed by phosphorylation of specific residues in LRRFIP2.