SPATA3 antibody (C-Term)
SPATA3
Reactivity: Human, Mouse
ELISA, WB, IHC (p), IF
Host: Rabbit
Polyclonal
unconjugated
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- Target See all SPATA3 products
- SPATA3 (Spermatogenesis Associated 3 (SPATA3))
- Binding Specificity
- C-Term
- Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SPATA3 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Specificity
- At least three isoforms of SPATA3 are known to exist, this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members
- Purification
- SPATA3 Antibody is affinity chromatography purified via peptide column.
- Immunogen
- SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3. The immunogen is located within the last 50 amino acids of SPATA3.
- Isotype
- IgG
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- Application Notes
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SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- SPATA3 Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- SPATA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Target
- SPATA3 (Spermatogenesis Associated 3 (SPATA3))
- Alternative Name
- SPATA3 (SPATA3 Products)
- Synonyms
- TSARG1 antibody, 1700011N12Rik antibody, 1700029H01Rik antibody, 4930424D10Rik antibody, Mtsarg1 antibody, Tsarg1 antibody, spermatogenesis associated 3 antibody, SPATA3 antibody, Spata3 antibody
- Background
- SPATA3 Antibody: The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.
- Molecular Weight
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Predicted: 21 kDa
Observed: 26 kDa - Gene ID
- 130560
- NCBI Accession
- NP_620712
- UniProt
- Q8NHX4
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