TRIM21 antibody (AA 290-340)

Catalog No. ABIN6991636

Product Details anti-TRIM21 Antibody

Target: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Binding Specificity: AA 290-340

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.

Purification: TRIM21 antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-TRIM21 antibody was raised against a peptide corresponding to 16 amino acids near the center of human TRIM21. The immunogen is located within amino acids 290-340 of TRIM21.

Isotype: IgG

Application Details

Application Notes: WB: 0.5-2 μ,g/mL, IHC: 5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIM21 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TRIM21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for TRIM21

Target: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Alternative Name: TRIM21 (TRIM21 Products)

Synonyms: RNF81 antibody, RO52 antibody, SSA antibody, SSA1 antibody, Ro52 antibody, Ssa1 antibody, tripartite motif containing 21 antibody, tripartite motif-containing 21 antibody, TRIM21 antibody, Trim21 antibody

Background: TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1). RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2). TRIM21 also acts as a cytosolic antibody receptor, detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3).

Molecular Weight: Predicted: 54kD

Observed: 54 kD kDa

Gene ID: 6737

NCBI Accession: NP_003132

UniProt: P19474